Disentangling the effect of photoperiod lenght, PPFD and DLI within a 24h diel cycle and under continous lighing, study of sugar status of lettuce plants

Vertical Farming is an innovative approach to food production, showcasing enhanced efficiency in water, fertilizer, and land utilization when compared to traditional cultivation methods. The absence of pesticides is a notable characteristic, and its global applicability minimizes food mileage, promoting local production, even in urban areas. The revolutionary aspect lies in the precise control of the light environment, a capability unattainable in open field or greenhouse production. Unfortunately, the profitability and sustainability of vertical farming encounter challenges due to high investment costs and energy consumption. The responses to DLI (Daily Light Integral), photoperiod, and PPFD (Photosynthetic Photon Flux Density) demonstrate intricate interactions that impact growth and quality. We have designed an experiment that encompasses various DLI levels, photoperiod variations, and PPFD challenges for lettuce. The objective is to investigate the trade-offs between PPFD and photoperiod or DLI. The aim of this MSc thesis is to identify indicators of carbohydrate accumulation that may function as a negative feedback mechanism to photosynthesis.

Used skills

• Carbohydrate analysis from samples collected at Climate chamber experiment
• HPLC analysis for carbohydrate, no previous experience needed

Interested in doing a BSc or MSc thesis at HPP? Please contact Katharina Hanika or Kim Vanderwolk via the HPP office (office.hpp@wur.nl).