PhD defence

Glycosylation of FIP-nha and its biological properties

PhD candidate	Y (Yusi) Liu
External promotor	Prof. Dr Harry J. Wichers
Co-promotor	dr.ir. S (Shanna) Bastiaan-Net dr. T (Tamara) Hoppenbrouwers
External copromotor	Prof. Dr Fengjiao Xin
Organisation	Wageningen University, Wageningen Food & Biobased Research, Wageningen Food & Biobased Research
Date	Wed 17 April 2024 13:30 to 15:00
Venue	Omnia, building number 105 Hoge Steeg 2 105 6708 PH Wageningen +31 (0) 317 - 484500
Room	Auditorium

Summary

The thesis studied the structure, characteristics and biofunction of FIP-nha, and how glycosylation impacts the structure and stability of FIP-nha, as well as the impact on FIP-nha’s anti-tumour activity via regulating innate immune cells (macrophages). We determined the crystal structure of rFIP-nha, and investigated the correlation linkage of structure and thermostability, and glycosylation impacts on hydrolysis resistance. Meanwhile, the immunomodulatory activity of rFIP-nha on macrophage polarization and tumour scavenging activity was investigated. In addition, the underlying mechanism was further explored, which contributes to a deeper understanding of FIP-nha’s anti-tumour activity.