Data underlying the publication: Interfacial protein adsorption behavior can be connected across a wide range of timescales using the microfluidic EDGE (Edge-based droplet GEneration) tensiometer

Samenvatting

# Introduction In the current study, we expand the use of our EDGE (Edge-based Droplet GEneration) device, and validate the findings with ADT (automated drop tensiometer) results at complementary timescales. Furthermore, we explore if the EDGE tensiometer could distinguish effects occurring at high whey protein concentrations (2.5 – 10 wt%). In this dataset, you can find an excel file with an overview of all dataset named "EDGEvsADT_WPI_20240516_v09_final.xlxs" (in the Analyzed_Data folder). You can also find the raw data in the Raw_Data folder with .txt files. Finally, in the Plotted_Data_Scripts folder you can find all processed data from the excel file in .txt extension together with a Matlab code to plot the data. # Abstract Hypothesis Our hypothesis is that dynamic interfacial tension values as measured by the partitioned-Edge-based Droplet GEneration (EDGE) tensiometry can be connected to those obtained with classical techniques, such as the automated drop tensiometer (ADT), expanding the range of timescales towards very short ones. Experiments Oil-water and air-water interfaces are studied, with whey protein isolate solutions (WPI, 2.5 – 10 wt%) as the continuous phase. The dispersed phase consists of pure n-hexadecane or air. The EDGE tensiometer and ADT are used to measure the interfacial (surface) tension at various timescales. A comparative assessment is carried out to identify differences between protein concentrations as well as between oil-water and air-water interfaces. Findings The EDGE tensiometer can measure at timescales down to a few milliseconds and up to around 10 seconds, while the ADT provides dynamic interfacial tension values after at least one second from droplet injection and typically is used to also cover hours. The interfacial tension values measured with both techniques exhibit overlap, implying that the techniques provide consistent and complementary information. Unlike the ADT, the EDGE tensiometer distinguishes differences in protein adsorption dynamics at protein concentrations as high as 10 wt% (which is the highest concentration tested) at both oil-water and air-water interfaces. # Measurements and data collection Measurements and data collection were done according to what is described in the paper We used two main setups and equipment, namely ADT (Automated Drop Tensiometer) and EDGE (partitioned-Edge-based Droplet GEneration) for data collection