Single-molecule Biophysics

The main focus of our lab is to study DNA-protein interactions on the molecular level. To achieve the necessary high temporal and spatial resolution, we use highly sensitive fluorescence-based techniques such as single-molecule FRET and super-resolution microscopy. In contrast to many ensemble-based experiments,  single-molecule experiments on biological objects can be performed under biologically relevant conditions and, more importantly, can reveal information about dynamic or static heterogeneity and the possible existence of sub‐populations.

In addition, we are interested in pushing the technical boundaries of single-molecule-fluorescence detection with nanofluidic devices and we are closely cooperating with the Laboratory of Biochemistry to study transcriptional regulators and plasma membrane steroid receptors in Arabidopsis.

See www.jhohlbein.com for more information.