Improved ice-binding proteins based on twist constrained helices

Summary

The invention relates to a protein comprising at least one ice-binding alpha helix, wherein the ice-binding helix has a twist of less than 100 degrees per residue, preferably a twist of less than 99 degrees per residue, most preferably a twist of about 98.2 degrees per residue. The invention further relates to a composition comprising such protein. The invention further relates to the use of said protein or composition as a cryopreservation agent, as a gas hydrate inhibitor or in coatings for de-icing materials. Lastly, the invention furthermore relates to a method for cryopreserving an aqueous mixture using said protein and a method for producing said protein.