PhD defence

Needles in a haystack: Pursuing directed evolution of CRISPR-Cas protein for improved genome editing

PhD candidate E (Evgenios) Bouzetos MSc
External promotor Prof. Dr John van der Oost
Co-promotor dr. RHJ (Raymond) Staals
Organisation Wageningen University, Laboratory of Microbiology
Date

Fri 4 October 2024 13:00 to 14:30

Venue Omnia, building number 105
Hoge Steeg 2
105
6708 PH Wageningen
+31 (0) 317 - 484500
Room Auditorium

Summary

CRISPR-Cas proteins have revolutionized genome editing. Nevertheless, optimization and tailoring of CRISPR-Cas systems are still necessary for optimal genome editing. In this thesis we pursued to develop new methods for the optimization of CRISPR-Cas proteins and applied already established methods aiming to improve a well-described Cas9 system. The described research finds significance in methods development and protein optimization and characterization.